Electrochemical Sensing of Interactions between DNA and Charged Macrocycles

In this work, we investigated aggregation of native DNA and thiacalix[4]arene derivative bearing eight terminal amino groups in cone configuration using various redox probes on the glassy carbon electrode. It was shown that sorption transfer aggregates surface electrode covered with black resulted changes electrostatic interactions diffusional permeability layer. Such alter signals ferricyanide ion, methylene green hydroquinone as to a degree depending their specific own charge. Inclusion layer independently confirmed by scanning electron microscopy, electrochemical impedance spectroscopy experiments doxorubicin model intercalator. Thermal denaturing affected charge separation interface probes. Using hydroquinone, less sensitive interactions, made it possible determine from 10 pM 1.0 nM (limit detection 3 pM) after min incubation. Stabilizers present commercial medications did not signal. The sensors developed can find future application assessment complexes formed macrocycles delivery agents for small chemical species.